Restriction fragment length polymorphisms of the mouse T-cell receptor gene families
نویسندگان
چکیده
منابع مشابه
Haplotyping the human T-cell receptor beta-chain gene complex by use of restriction fragment length polymorphisms.
We have studied the genetic segregation of human T-cell receptor beta-chain (TCR beta) genes on chromosome 7q in 40 CEPH (Centre d'Etude du Polymorphisme Humain) families by using restriction fragment length polymorphisms (RFLPs). We constructed haplotypes from eight RFLPs by using variable- and constant-region cDNA probes, which detect polymorphisms that span more than 600 kilobases of the TCR...
متن کاملMultiple restriction fragment length polymorphisms of the human epidermal growth factor receptor gene.
We have examined the epidermal growth factor (EGF) receptor gene for structural alterations in fresh human tumors. DNA samples from 92 patients with solid tumors (lung cancer, 37; breast cancer, 24; head and neck cancer, 17; other tumors, 14) were analyzed and compared with those from 22 leukemia patients and 14 individuals without malignant neoplasms. When DNA samples were digested with HindII...
متن کاملMan Using Restriction Fragment Length Polymorphisms
We describe a new basis for the construction of a genetic linkage map of the human genome. The basic principle of the mapping scheme is to develop, by recombinant DNA techniques, random single-copy DNA probes capable of detecting DNA sequence polymorphisms, when hybridized to restriction digests of an individual's DNA. Each of these probes will define a locus. Loci can be expanded or contracted...
متن کاملNeisseria gonorrhoeae by restriction fragment length polymorphisms
Objective-To characterise Neisseria gonorrhoeae isolates by restriction fragment length polymorphisms (RFLPs) in ribosomal RNA genes. Design-Generation of RFLP patterns by HincII restriction of rRNA genes followed by hybridisation with a nonradioactive labelled broad spectrum 16 + 23S rRNA gene probe. This typing method was developed and compared with MAb based serotyping. Specimens-Forty three...
متن کاملRestriction fragment length polymorphism of the gene encoding the alpha chain of the human T cell receptor
Two allelic forms of the T cell antigen receptor alpha chain gene were discerned by restriction fragment length polymorphism (RFLP) employing the T cell antigen receptor alpha chain probe pGA5, and the restriction enzyme Bgl II. Analysis revealed that the polymorphic fragments are detected by a probe specific for the constant region exon of the T cell antigen receptor alpha chain gene. Furtherm...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Immunogenetics
سال: 1989
ISSN: 0093-7711,1432-1211
DOI: 10.1007/bf02421215